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Originally Processed With FOIA(s): FOIA Number: S FOIA MARKER This is not a textual record. This is used as an administrative marker by the George Bush Presidential Library Staff. Record Group/Collection: George H.W. Bush Presidential Records Collection/Office of Origin: Speechwriting, White House Office of Series: Aarhus, Carol, Files Subseries: Alpha File, 1990-1992 OA/ID Number: 13866 Folder ID Number: 13866-007 Folder Title: Science and Technology Stack: Row: Section: Shelf: Position: G 19 2 5 7 02/28/1991 13:09 FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.01 I rec'd this too late ive to include in the Westinghouse speech - 1991 Ms. Carolyn Cawley, passing it around for Office of Communications, The While House, anyone to use in the Washington D.C. 20500 next science or volunteer Fax No: (202) 456-6218 or education speech -CC Dear Ms. Cawley, Earlier this week, my daughter Rowan and I met with the publisher of 'Fortune' magazine, Mr. James B. Hayes, to discuss a new industry- college volunteer program called SMArT, for the Science and Math Achiever Teaming program, that she is piloting at Yale in the New Haven schools. Mr. Hayes suggested that the White House might be interested in this program as a part of its Points of Light program, because it addressed the twin national needs, as he saw them, for more volunteers and for an effective, business-led, method of 'going national' with a program to fulfil the President's pledge for the U.S. to lead the world in this area by the next decade. Rowan, as an ex-Westinghouse winner, was going to be in Washington D.C. this weekend, March 2 & 3, for the 50th Anniversary Science Talent Search Awards, and she knew the President would be involved in the STS functions. She therefore thought she should let the Science Advisor, Dr. Bromley, know about SMArT, because we had submitted an article to 'Fortune' in the form of an open letter to the President and because the White House might be interested in this initiative. I faxed her letter and FedExed the material to Dr. Bromley's office yesterday, but, in following up today, found that it hadn't reached anyone yet. Accordingly, I contacted your office and, per your office's request, am faxing herewith her letter, the draft article and a Yale Daily News article on the New Haven/Yale pilot program she is running for your information. I have also faxed this material to Ms. Janice Howell at the Office of Science and Technology Policy. If you feel the White House may be interested in this, please give me a call at (815) 226 7913 (business) or (815) 397-0584 (home) and I would be pleased to discuss it further with you. Thank you for your interest. Yours sincerely, John G. Lockwood FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.02 Scientists 02/28/1991 13:09 Help Youngsters Get SMArT By Bob Datta from professors and scientists who in order to do real science you must she said. will let the students visit their labs YON Staff Reporter have five years of study," she said. She spent most of her summer and workplaces. "I think that you learn the most as working closely with the New The students will work in all Rowan Lockwood '93 wants you go along, picking up skills Haven school board and with kids to get SMArT. sorts of fields, ranging from solar along the way" Dwight Hall to get SMArT rolling, Lockwood's efforts don't involve power to DNA research to Bruce Guenin, a scientist at Olin she added. Max and Agent 99, but the Science dinosaurs and evolution. using the and a volunteer for SMArT said the Jack Hasagawa, coordinator of and Math Achiever Teaming pro- lab space at Troup. program will do much more than Dwight Hall helped her to design gram. SMArT, which will begin "I want the kids to get a good make the kids interested in science. SMArT this summer. "I know that next semester, will allow students at view of what science really is, and "It enhances their self-esteem if unless someone does something Troup Middle School to research know what it is all about," Lock- people are interested in them," he about getting children interested in wood said. fundamental science with help from said, adding that industry should math and science, American society Yale student mentors, Yale profes- Students are frequently turned play a role in New Haven education will go down the tubes," be said. sors and New Haven industrial sci- off from science by bad textbooks, "10 give kids an 1dea of the skills Kasagawa pointed to recent entists. or "A single lousy math or intro-sci- they have, and need, to succeed" demographic surveys which indi- Twice a week after school, the ence class." she said, adding that Lockwood said the idea for cate that American children get Yalies will visit Troup to help the hands-on experience can teach stu- SMArT came from ber experiences good science grades until the fifth dents as much as can classroom students design and reseach their in high school. "I did research at a grade, when a "botleneck" occurs. time. own science projects. The Troup local college, published a paper, and "At the upper levels of eduation. students will also receive guidance "There is this misconception that I learned lot from the experience," See SMArT, Page 4 , ( / a b Γ. a $ f 1 B e :- re is d / / D Marian Harris '93 and Rowan Lockwood '93, co-coordinators of the is Science and Math Achiever Teaming program plan how to get middle 0 school students interested in science. & Students, Scientists t Work With City Youth SMArT, from Page 1 of SMArT, concurred. "The kids' there is a devaluation of math and attention span will determine how : science in our schools," be said. much work they do," she said. "The The program will begin in Jan- projects will, for the beginning, uary, when the second semester at only last a semester. It will give the $ Troup begins. For the first week, kids a feeling of getting something the SMArT student-volunteers will done," Harris said. 1 present a "smorgasboard of sci- The Troup school already has ence" to offer the kids the broadest pretty good science facilities, Lock- e possible picture of what they can wood said, because it is a math-sci- do. ence magnet school for the sixth The students themselves, with grade, anid because NASA gives # little. guidance from theis SMArT resources for its Young Astroneut mentors, will then choose the sort program. 1 of project they would like to do. SMArT is be partially funded by "This program is, and has to be, the Howard Hughes grant, which is 02/28/1991 13:10 FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.03 P.O. Box 2285 Yale Station New Haven CT 06520 February 26, 1991 The Hon. D. Allan Bromley, Assistant to the President for Science and Technology, Old Executive Office Building, 17th. St. & Pennsylvania Ave. N.W., Rm. 358, Washington D.C. 20506 Dear Dr. Bromley, Earlier this week, I met with the publisher of 'Fortune' magazine, Mr. James B. Hayes, to discuss a new industry/college volunteer pilot program we have started in the New Haven Troop school, which is funded by the Hughes grant. It is called SMArT, for the Science and Math Achiever Teaming program. This weekend, I will also be attending, as a 1988/89 Westinghouse alumnus, the Westinghouse Science Talent Search Reunion in Washington D.C. on the occasion of the 50th STS Awards, where I am also hoping for an opportunity to make a presentation on SMArT. Given your Office's interest in science and mathematics education and the efforts this Administration is making to encourage volunteerism through the Points of Light program, I thought that you may be interested in this program. I am enclosing an article my father and I have written on it and submitted to 'Fortune', along with materials on the New Haven/Yale pilot program. The program was jointly devised by my father and me. I drew on the latent interest I thought college science students would have in increasing their hands-on involvement in science. My father, who works in international business development and innovation for a company in Rockford IL., drew on the franchising techniques of the Junior Achievement organization that he felt strongly had to be applied to math and science if an effective national effort was to be mounted. ... 2 02/28/1991 13:11 FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.04 I am very grateful for his interest to Mr. Hayes, who was, I think, enthused by both ideas; the first that college students could supply the numbers of volunteers that are needed but are lacking in industry, and the second that SMArT could be 'seeded' rapidly and effectively throughout the nation, using incentivization, formularization, and franchising business techniques. I am also grateful to Dean Kagan, Dean Judith Hackman and Jack Hasagawa at Yale, who have been very supportive of the program. If you are interested in following up on this, my telephone number at Yale is 203/436-0801. However, it may be easier to contact me through my father, Mr. John Lockwood, at my home address, 1124 Post Drive, Rockford, Il 61108, (tel. 815/397-0584), or at his business telephone, 815/226-7913. During my stay in Washington this weekend, March 2 and 3, I will be staying at the Washington Hilton 202/483-3000. Thank you for your interest in the program and for your time. Yours sincerely, Rowan Lockwood Rowan Lockwood (Ms.) P.S. We have changed the front page of the attached article from the copy we faxed earlier. I regret any inconvenience. 02/28/1991 13:11 FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.05 13/44171 FRANCHISING SCIENCE AND MATH ACHIEVER TEAMS - A SMArT GAMEPLAN, MR. PRESIDENT. C John G. Lockwood, 1124 Post Drive, Rockford, IL 61108, and Rowan Lockwood, Box 2285, Yale Station, New Haven, CT 06520, 1990 Telephone: Bus. (815) 226-7913 Home (815) 397-0584 02/28/1991 13:11 FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.06 The State of the Union addresses of the past two years have been long on educational goals but short on specifics. of particular interest to business is just how our students are supposed to lead in the big world league of math and science by the year 2000? Clearly Mr. Bush wants to coach a team effort, with business a prime player, but where's the drawcard for the kids? Where's the game plan? Commentators place long odds on a win whatever the gameplan. To them, simply increasing science education spending will not stem the decline in numbers and skills of teachers and students alike. The problems are universal, the solutions piecemeal. To meet Mr. Bush's goal, a national classroom program must reconcile too many institutional interests in government and the educational establishment in too short a time. Always pitching short, the experts leisurely walk the players. Outside the classroom, however, at the local level, science education is more fun than a pickup game at recess. All over the U.S., unsung but effective, ever more players - - industry volunteers as mentors, college students as tutors - are inventing ever more plays to help out - science clubs and fairs, enrichment and application programs, internships, field trips. The rush of industry support could fill a grandstand. Outside the classroom, it is clear that opportunity and achievement, those uniquely American values that once built the little red schoolhouse and made Johnny run, still endure. But to win in ten years, the U.S. needs a drawcard, a gameplan and a players league at the national level. One drawcard with that poten- tial is the opportunity for kids to try out in science offered by the Science and Math Achiever Teaming (SMArT) program being introduced at Yale for New Haven schools, funded by a Hughes grant. Its gameplan is to build early achievement in science: its 'league' will be franchised community volunteer teams with their own organization and awards. SMArT aims to motivate middle and high school pupils in science and math through early achievement in original, long-term, research pro- jects, supported by volunteer teams from industry and academia. Team techniques, as developed, will comprise a rulebook for franchising new teams. Teams will be sponsored by industries and colleges cooperating with the pupils' schools, and, importantly, will be open to all comers. 02/28/1991 13:12 FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.07 Offering opportunity to all in science is important. Early effort counts more than perceived ability. Science programs have to look hard at their mission through kids' eyes or risk attracting only those already drawn to science. SMArT wants the many with potential in science to try out, not just the few committed to it. Science is one discipline, (languages another), where a hard grind sharpening the tools of learning usually precedes any fun in using them. Its future in the U.S., now the baby boom is over, depends on motivating the many. Learning by doing is also key to math and science. If achievement is to spark motivation in those subjects, then it must be meaningful, and result from long-term, original research projects that require more effort for longer than, say, the average science fair project, and correspondingly reward participants with a greater sense of discovery. Finally, achievement, to thrive in science as in life, must start with taking control and end with recognition. All SMArT projects will be chosen and controlled by the junior team members and will be eligible for local incentive awards and entry in national competition. The SMArT guidelines reflect these concepts: Projects will be devised and managed by the school pupils and must involve original or creative work in results or methodology. Projects will be not less than one school year in duration and require a pupil/parent written commitment to timely completion. Industry volunteers will commit to provide project guidance, research resources and logistics support for the students involved. College volunteers will commit to provide hands-on help to, and pursue sources and technical resources for, the school pupils. Cooperating industries will provide schools liaison, project resource, logistics support, and local incentive awards. Cooperating colleges will provide project source access, staff advisory services and incentives complementing the industry awards. A national center will provide organizing help, project resource, program research and a state and national awards structure. 02/28/1991 13:13 FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.08 The core SMArT concept is its three-way, volunteer-based teaming of schools, industry and colleges. This expands the resources needed for an effective program and creates important complementary benefits. Colleges can directly address their declines in enrollments and graduations in math and science while their students take advantage of volunteer service, job opportunities, hands-on research and teaching experience well beyond the lectureroom and lab. Industries and universities can cooperate more in recruiting, innovation and contract R & D, while contributing significantly to competitiveness. Industry volunteers will find working with youth recharges their on-the-job energies, and, should they flag, the college students can bring their own youthful enthusiasm to bear. Science teachers can have some respite and their pupils can have a foretaste of their college and workplace futures. Even the local science fair now dreaded alike by students without ideas, parents without time and teachers without help, - can become an opportunity instead of a chore. In 14,000 schools, a national model for this program already exists. Junior Achievement, the leading business-education partnership in the country, has drawn students to business for over seventy years. The name Junior Achievement came from the original JA concept of business volunteers helping students achieve success by starting their own businesses on their own time. Millions of parents know how effective that wellspring of achievement has been in motivating their children. Equally effective but less well known has been JA's pioneering use of that quintessentially American business tool, franchising, to expand its winning concept across the U.S. In this, JA has not only played to an American business strength but has demonstrated the potential in private bodies 'going national' with innovations in education. The SMArT gameplan draws on the same national strengths, opportunity, achievement, volunteerism in higher education and in business to offer a real chance at a come-from-behind world victory in science and math. How about a tryout then, Mr. President, Coach, Sir? Call on the heavy hitters on your science team from industry and academia. How about a tryout for SMArT as America's farm team in the big league of world science and math education? 02/28/1991 13:13 FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.09 ROWAN LOCKWOOD, (Ms.), Home address: 1124 Post Drive, Rockford, Illinois 61108, U.S.A. Home tel.: (815) 397 0584. Birthdate: 7 June 1971 EDUCATION 1989-91 Attending Yale University, New Haven, Connecticut, U.S.A. My first year courses primarily consisted of the inter-disciplinary Directed Studies Program (Western civilization philosophy, history and literature), but also included Evolutionary Biology and Chemistry. My second year studies have included Mineralogy, Geology, and Anthropology courses, directed towards a double major in Geology and Anthropology, along with History and French. 1986-89 Charter Class Member, Illinois Mathematics and Science Academy (IMSA), 1500 W. Sullivan Rd., Aurora, IL 60506-1039, U.S.A. IMSA is a three-year, residential, state-supported high school with competitive admission for all Illinois sophomore-level students. 1985-86 Completed Grade 9, Rockford East H.S., Rockford, IL. ACADEMIC ACHIEVEMENTS 1989-91 - Publication of paper, "Evidences of Bipedalism in (Larger) Pterosaurs Derived from a Biomechanical Methodology" in BASE journal, Vol. 8 No. 1, (Spring, 1990) - Admitted to the Yale Directed Studies Program, (1989) 1986-89 - Winner, the Illinois State Academy of Science Frank H. Reed Award, best Westinghouse report, (May, 1989) 1 Winner, 48th. Annual Science Talent Search for the Westinghouse Science Scholarships, (January 1989). - Presented abstract at the annual meeting of the Society of Vertebrate Paleontology at Drumheller, Alberta, Canada, (Oct. 1988). - Interned in the Dept. of Paleobiology, the National Museum of Natural History, Washington D.C. (Aug. 1988). - Presented INTECH 88 paper to the DOE location research team for the U.S. Super Collider facility, (May 1988). - Won First Prize, the INTECH 88 Science Competition for Chicago High Tech Corridor Area Schools and the American Nuclear Society (Chicago Section) Award for an energy- related project, (May 1988). - Admitted to the Charter Class of the Illinois Mathematics and Science Academy, (1986). - Rockford East High School Academic Honor Roll (1985-86). 02/28/1991 13:14 FROM SUNDSTRAND CORP. HDQTRS. TO 8 5094 91 2024566218 P.10 ATHLETICS & EXTRACURRICULAR PARTICIPATION 1990-91 - Won Dartmouth Diving Meet 1M & 3M Events (Feb. 1991) 1989-90 - Varsity Letter, Yale Swimming & Diving Team. - Won Harvard-Yale Diving Meet 1M Event (Dec. 1989) 1988-89 - 17th. place, Illinois High School Association (IHSA) State Diving Meet, (Nov. 1988). - Won IHSA W. Chicago Sectional Diving Meet, (Nov. 1988). - Captain, IMSA Diving Team. - Section Leader (Flutes), IMSA Concert Band. - Cast Member, IMSA Drama Club 1989 production. 1987-88 - 5th. place, Scholastic Women's 1M Diving Competition, Prairie State Games, (July 1988). - 18th. place, IHSA State Diving Meet, (Nov. 1987). - 2nd. place, IHSA Waubonsie Valley Sectional Diving Meet, (Nov. 1987). - Certification in Advanced Lifesaving and CPR. 1986-87 - 5th. place, IHSA Waubonsie Valley Sectional Diving Meet, (Nov. 1986). 1985-86 - Elected Student PE Leader for Sophomore year. - NASTAR Silver medal in skiing, (April 1986). COMMUNITY WORK SERVICE 1990-91 - Conceived and initiated the New Haven/Yale Science and Math Achiever Teaming (SMArT) pilot program at Troop public school in New Haven. SMArT is a volunteer program designed to encourage students in math and science and funded by Yale from the Hughes Foundation. 1988 - Volunteer lifeguard at Rockford College pool, Rockford, IL, for summer swims for children and the handicapped. 1986-88 - IMSA work-service, including Foreign Language Department assistant, lifeguarding and other duties. PERSONAL STATEMENT At Yale, I am pursuing a double major in Geology and Geophysics and in Anthropology. However, I would like to supplement these courses by undertaking interdisciplinary work involving paleoanthropology and paleontology. PUBLICATIONS & PRESENTATIONS "Evidences of Bipedalism in (Larger) Pterosaurs", (with Dr. Virginia Naples, Northern Illinois University), presented at the 48th. annual meeting of the Society of Vertebrate Paleontology at Drumheller, Alberta, Canada, October 1988; abstract published in the Journal of Vertebrate Paleontology, Supplement to No. 3, Vol. 8, Sept. 23, 1988. THE WHITE HOUSE WASHINGTON February 15, 1991 TO: ADMINISTRATION OFFICIALS FROM: OFFICE OF PUBLIC AFFAIRS 195 OEOB, 202/456-2483 For your use and distribution. PRESIDENTIAL WIRE February 15, 1991 On Science and Math Education President Bush's remarks to the American Association for the Advancement of Science "Technology may be the key to the future, but people are the key to technology. The national education goals that we established with the nation's governors explicitly recognizes this connection. One of our most ambitious goals is for American students to be first in the world in science and math achievement by the year 2000. "Our budget includes substantial funding increases for math and science education. But those math and science goals will never be achieved if they are seen simply as goals for government alone. All sectors of society must recognize the importance of scientific literacy and strive to achieve it." "Last fall, we had 200 of the best mathematics and science teachers in the country here to the White House. And more than a few of those teachers pointed out that kids are natural-born scientists. They delight in the sheer pleasure of learning new things, making something work, understanding the world." "Sharing science's sense of adventure through education and outreach has never been more important that now." Washington, DC February 15, 1991 For more information please contact the White House Office of Public Affairs at 202/456-2483. NIST UNITED STATES DEPARTMENT OF COMMERCE NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY Covers. These micrographs were Below. Recognizing the critical produced using a new type of role of quality to business, optical surface imaging- Congress created the Malcolm scanning scattering micros- Baldrige National Quality Award copy-that can be used to in 1987. Named after former monitor and measure changes in Secretary of Commerce surface structure and micro- Malcolm Baldrige, the award topography with nanometer honors American companies height sensitivity. For example, that achieve the highest level it can measure and image the of total quality management. surface roughness of polished Presented annually, the award semiconductor wafers and is intended to help motivate detect individual surface U.S. companies-both large defects on these wafers. and small-to improve their total quality management, including the quality of their products and services. NIST manages the award program with the private sector. NIST at a Glance The National Institute of Standards and Technology (NIST) was established by Congress "to assist industry in the development of technology needed to improve product quality, to modernize manufacturing processes, to ensure product reliability, and to facilitate rapid commercialization of products based on new scientific discoveries." A principal agency of the Commerce Department's Technology Administration, NIST has as its goals: to aid U.S. industry through research and services, to contribute to public health and safety, and to support the U.S. scientific and engineering research communities. NIST conducts basic and applied research in the physical sciences and engineering, developing measurement techniques, test methods, standards, and related services. The Institute does generic and precompetitive research and development work on new advanced technologies. Sites Gaithersburg, Md. (headquarters) Boulder, Colo. Budget $350 million (est. all sources, 1990) Staff 3,000 scientists, engineers, technicians, and support personnel, plus some 1,000 visiting scientists each year Main Chemical science and technology Research Physics Areas Materials science and engineering Electronics and electrical engineering Manufacturing engineering Computer systems Building technology Steuben Glass Fire safety Computing and applied mathematics A National Resource The competition is intense: Make products that are better, less expensive, and more reliable-and get them to market first-or lose out in the world marketplace. At the National Institute of Standards and Technology, we provide U.S. in- dustry with many of the tools it needs to compete in world markets. NIST techni- cal services and research programs are helping companies become more effi- cient, more productive. The Institute's staff are working with hundreds of firms of all sizes to build quality and innova- tion into their operations. And we are stepping up our efforts to help ensure that U.S. industry can take commercial advantage of emerging technologies. Strategic partnerships-sharing costs and risks-are essential in today's tough marketplace. We know coopera- tion pays. Since its founding in 1901 as the National Bureau of Standards, the Institute has teamed with thousands of organizations. NIST-pioneered innovations are incorporated in nearly every line of products in our economy. NIST is a national resource. | invite and urge you to use our research and NIST, Gaithersburg, Md. The services. Institute encourages the use of its research and testing facili- ties by industry, government, John and academia for cooperative and proprietary research. Among the facilities at the Gaithersburg site are: a John W. Lyons 20-megawatt research reactor Director with a cold neutron source, a synchrotron ultraviolet radiation facility, a metals processing laboratory, an automated manu- facturing research facility, a fire research facility, and an open systems interconnection (OSI) security laboratory. NIST researchers are developing new techniques to help improve building materials. One such technique is a computer model that can simulate how the microstructure of concrete develops during the setting process. Models like this ulti- mately will be used to predict characteristics of concrete, such as performance, strength, and durability. P.I.X.A.R NIST has constructed the first U.S. facility devoted to cold neutron research, which will give U.S. industry prime ac- cess to one of the key tools of modern materials science. The 15 experimental stations being installed at the facility will be available for use by outside organizations. For example, the neutron depth profiling (NDP) instrument (near right) is believed to be the most power- ful dedicated NDP instrument in the world. Using NDP, researchers are able to make accurate measurements of impurities and dopants that greatly affect the properties and performance of new mate- rials used in semiconductors and other high-technology products. Donald Becker NIST: A World-Class Partner NIST contributes to the advance of tech- nology through world-class science and engineering research. Measurements, testing procedures, quality assurance methods, and innovations developed at NIST have helped build the technical infrastructure upon which much of the U.S. economy rests. Virtually all U.S. industries have bene- fited-from machine tool to food proc- essing to semiconductor manufacturing, and from aerospace to construction to biotechnology. Studies have opened new lines of research, led to new scientific in- struments and manufacturing methods, and helped open new markets. Examples range from neon signs to closed caption- ing on television, from atomic clocks to radio direction finders for planes. NIST accomplishments are often "the story behind the story," leading to im- provements in measurement capability In lightwave technology, elec- and, ultimately, efficiency, reliability, and trical energy is converted by quality. lasers to lightwaves, which are sent through hair-thin strands of glass. This process is used Building on its technical expertise, NIST in compact disk recordings and optical character readers and provides valued, neutral leadership in will play a larger role in com- munications in the future. For national and international standards the potential of optical com- arenas. Staff members chair about munication to be fully realized, optical devices must be turned 10 percent of the 1,300 voluntary in- on and off at the rate of a tril- dustry standards committees on which lion times a second. NIST researchers are using lasers they serve. They help achieve consensus in a program to measure the response speed of optical com- among often fragmented business inter- munication devices. ests, furthering the competitive position of U.S. industry in world commerce. NIST researchers played a lead- ing role in the development of the microanalysis technique- compositional mapping-that uses digital computer tech- nology coupled with electron beam instruments to "map" the distribution of chemical ele- ments on sample surfaces. Micrometer-scale analyses are important in characterizing new, high-technology materials such as aluminum-lithium alloys, high-temperature superconduc- tors, and semiconductors. In the ceramic material at right magne- sium is black, vanadium blue, and cobalt green. In a leading-edge experiment, NIST researchers laser-cooled a mercury ion, confined in a radio frequency "trap," to its funda- mental limit. The work is impor- tant for spectroscopy, a study of the nature of matter through various radiation it emits. The result may be a highly sensitive spectrum analyzer as well as the basis for a new standard of timekeeping. Geoffrey Wheeler Since the early 1970s, NIST has been developing cost-effective ways to help protect computer- ized data. These methods include sound management practices as well as technical solutions. NIST has devised a prototype system for controlling access to a computer system that uses a password, a smart card, a fingerprint reader, and cryptography. *51711 IDENTIA PR NIST Intel WYSE Above left. Chlorofluorocarbons Right. NIST provides the semi- (CFCs)-a family of chemicals conductor industry with the used widely for refrigerants, measurement methods and tech- foam in building insulation, nology necessary for the manu- furniture, and car seats-are facture of reliable, high-quality breaking down the ozone layer, chips. In one case, at the re- which protects the Earth from quest of industry, NIST re- harmful levels of ultraviolet searchers developed the radiation from the Sun. NIST technical basis for three test scientists are measuring the methods for evaluating elec- chemical and physical proper- tromigration, a serious failure ties of alternative refrigerants mechanism in semiconductor to help industry find effective integrated circuits. replacements for CFCs. For example, a new database called REFPROP, issued by NIST through its standard reference data program, gives refrigera- tion engineers, chemical and equipment manufacturers, and others a new research tool for evaluating the performance of refrigerants and refrigerant mixtures. Semiconductor Services NIST Research: A Strong Foundation The research of NIST scientists and engineers places them at the frontiers of advanced technology. Advanced semi- conductor devices. Optoelectronics. Superconductors. Advanced materials- ceramics, composites, polymers, new alloys, and thin films. High-density data storage. Advanced computing systems. Digital imaging technology. Bio- technology and bioprocessing. NIST specialists are developing tech- niques and instruments that will ulti- mately determine whether technological promise becomes commercial reality. For example, they are working to ensure that U.S. firms will be well prepared to vie in the world market for biotechnology products-predicted to total $40 billion by the year 2000. NIST is helping to lay the foundation for commercial-scale pro- duction of bioengineered compounds. Working with industry and Much of NIST's R&D effort lies at the in- the Navy, NIST researchers designed and assembled a creasingly fuzzy boundary between basic state-of-the-art flexible manu- facturing system for the Navy and applied research, where commercial shipyard at Mare Island, Calif. spinoffs can follow on the heels of This workstation (inset) is capable of operating, largely discovery. The continuing miniaturiza- unattended, 24 hours a day, tion of electronic devices and the build- producing parts on demand for nuclear submarines. Many of ing of new materials atom by atom are the advanced techniques used in the workstation were devel- two such areas. oped in NIST's Automated Manu- facturing Research Facility Today, NIST studies of "laser-trapped" (AMRF). The AMRF is an experi- mental facility designed to atoms or of the changing behavior of study the application of advanced robotic and computer- molecules as they grow a few atoms at a control techniques to small- time are enhancing scientific under- batch manufacturing. Pictured here is the cleaning and debur- standing. Tomorrow, this knowledge ring workstation. could be embodied in new products. Researchers from NIST and the 502 Aluminum Association have developed a process control sensor (right) that rapidly meas- SHIFT ures the internal temperatures of extruded aluminum as it is produced. The sensor, which ---------- has performed successfully at plant trials, should help alu- / minum manufacturers optimize the quality and production of extruded aluminum. The sensor is being modified for use in other types of aluminum pro- duction, and can be adapted to the production of other metals, including steel (above). NIST has organized a consor- tium of industry and government researchers with the goal of developing a prototype auto- mated analytical chemistry laboratory. When completed, the modular system will perform the three major steps of a chemical analysis: sample prep- aration (dissolving by micro- waves, as shown in the photo to - the left), separation, and detec- tion. Though automation has permeated numerous U.S. in- dustries, the analytical labora- tory has lacked the tools to incorporate automation. The consortium aims to change this by producing a generic labora- tory that can be adapted readily by members for their individual needs. Each day more than 250 million chemical analyses, at an annual cost of $50 billion, are made in this country in fields such as medicine, the en- vironment, and food products. Thousands of biological tissue samples and environmental specimens are being preserved in the NIST Biomonitoring Speci- men Bank for future analysis. The bank is especially valuable for determining pollutants pres- ent in the environment at a given place and time. Diamond-tool turning and grind- ing machines are the acme of precision manufacturing tools, capable of machining high- precision optical finishes without additional polishing (such as on this copper mirror for a laser system). NIST re- searchers and their indus- trial partners are working on improved methods of monitoring and controlling diamond turning machines to improve the preci- sion and production of highly efficient optics such as mirrors for laser welders. NIST researchers have joined with industry to develop the measurement methods and scientific basis for process monitoring sensors, control models, and other tools manu- Below. A laser and radio- facturers need to increase the chromic sensors form the basis speed and reliability with which of a NIST-designed system that polymer composite materials could be used to monitor, in are processed. As part of this real time, a number of widely effort, model materials with used industrial radiation special fiber surface treatments processes, such as polymer are prepared (left) to study the curing, sterilization of medical effect of process changes on devices, or semiconductor hard- composite performance. ening tests. Left. Computer models devel- oped by NIST researchers make it possible to duplicate real fires without having to burn a room or building. One such model, called HAZARD I, makes it possible to predict the spread of smoke, toxic gases, and heat from a fire in a room to other parts of a building. These mod- els give engineers, architects, building owners, and others the knowledge to improve fire safety at reduced costs. the Left. Integrated Services Digital Above. More accurate measure- Network-ISDN-is a telecom- ments of cholesterol in food are munications technology used to possible using the standard ref- send and receive voice, data, erence material developed by and pictures simultaneously researchers from NIST, the Col- over digital telephone lines, lege of American Pathologists, which are increasingly high- and the U.S. Department of Agri- capacity fiber-optic transmis- culture. For example, this mate- sion systems. NIST is working rial was used as the quality with both manufacturers and control material in a nationwide prospective users of this tech- study of the cholesterol content nology to ensure that ISDN prod- of eggs. The study showed pre- ucts and services from different viously accepted values to be in manufacturers and vendors are error, and new, more accurate compatible and that they meet values were determined. users' needs. Cooperation, Naturally Results-oriented cooperation. That has always been NIST's way of doing busi- ness. The Institute's focus on industrial problems was the catalyst for some of the nation's first research consortia. Since the 1920s, industrial researchers have been coming to the Institute to use its vast array of research instruments and testing equipment and to work with its technical specialists, many of whom are the recognized leaders in their fields. Now, through cooperative research and development agreements, companies can be assigned the rights to intellectual property resulting from collaborations. And from the start, firms have depended heavily on NIST technical services. Ninety of the nation's 100 most R&D- intensive firms now use NIST measure- ment or data services; 27 of the top 50 have placed researchers at the Institute during the last 10 years. In all, NIST staff members are involved in over 1,100 The NIST-developed near-field scanning technique to char- collaborations. Any U.S. firm-large or acterize high-performance microwave antennas used for small-is eligible to collaborate with communication, radar, and NIST on projects of mutual interest. navigation has revolutionized the measurement of antenna performance. The near-field Research results-disseminated method predicts the signal that will occur kilometers from an through an annual output of 3,000 antenna by making careful publications and technical talks and measurements very near it. The NIST near-field antenna meas- more than 100 major, Institute-hosted urement facility, located in Boulder, Colo., is available for conferences and workshops-have use on either a cooperative or concrete applications, either today or reimbursable basis by industry, government, and academia. tomorrow. Geoffrey Wheeler 14 Left. This NIST cone calorimeter provides data critical to predict- ing the fire hazard of a product using a small sample of mate- rial-replacing time-consuming and expensive full-scale tests. The U.S.-based ASTM has adopted a voluntary fire hazard test method based on the instru- ment. It also is being con- sidered as the basis for a standard test method by the International Organization for Standardization. Wide World Photos, Inc. Left. NIST scientists are Above. Investigating the developing the measurement in- October 1989 Loma Prieta earth- formation industry needs to pro- quake in California, NIST re- duce diamond films with many searchers concluded that deep, of the properties of natural dia- unstable soil deposits played a mond. The physical and chemi- major role in the damage to cal properties of diamond make buildings and "lifelines"- it a highly desirable material for bridges, highways, and water aerospace products, electron- and gas pipelines. As part of ics, and industrial equipment. the National Earthquake The goal of the NIST research is Hazards Reduction Program, to characterize the production NIST conducts research and pro- processes, the structure, and vides technical support to the other properties of diamond private sector and government films to help industry produce agencies working to improve high-quality, high-performance the performance of buildings advanced materials. and other structures subjected to earthquakes. 1 $ DEPARTMENT or COMMERCE 325 BROADWAY For More Information Chemical science and technology Harry S. Hertz A309 Chemistry Bldg., 301/975-3145 Physics Katharine B. Gebbie B160 Physics Bldg., 301/975-4201 Materials science and engineering Lyle H. Schwartz B309 Materials Bldg., 301/975-5658 Electronics and electrical engineering Judson C. French B358 Metrology Bldg., 301/975-2220 Manufacturing engineering John A. Simpson B322 Metrology Bldg., 301/975-3400 Computer systems James H. Burrows B154 Technology Bldg., 301/975-2822 Building technology Richard N. Wright B250 Building Research Bldg., 301/975-5901 Fire safety Jack E. Snell A247 Polymer Bldg., 301/975-6850 Computing and applied mathematics Francis E. Sullivan A438 Administration Bldg., 301/975-2732 Technology services Donald R. Johnson A363 Physics Bldg., 301/975-4500 General inquiries Peggy M. Saunders E128 Administration Bldg., 301/975-3058 Note: All addresses at NIST, Gaithersburg, Md. 20899 NIST COULTER Photo ppy.inteservation HEMATOLOGY IMMUNOLOGY FLOW CYTOMETRY COMP FINE CHILL II Coulter. The Search Continues. 150 Fig. 26 CH2 25 CH GH, 50,150 200 150 150 0'S- X=LYF Y=PR 0 KODAK TX 50 TX 50 "CH "Cn2 18 12 CH H CH2 5 13 H 24 25 11 19 C C CH D H 14 27 C 11 Hii C A B H 29 7 CH₃ FL Introduction Millions of tests are performed every day - all over the world - on COULTER® equipment. In hospi- tal, clinical, research, and industrial laboratories, COULTER instruments provide vital information. Diagnos- ing disease, controlling the quality of pharmaceuticals and cosmetics, measuring air contamination and moon dust are but a few of the many applications. Doctors, medical technologists, researchers and sci- entists have relied on COULTER instruments for over thirty years. The CoulterPrinciple-WallaceH Coulter's discovery for counting and sizing microscopic particles — is the basis for that trust. The Principle became a family of companies the Coulter corpo- rations. A leader in technological innovation, Coulter produces prod- ucts and services for patient care, scientific, research and industrial applications. Coulter- a worldwide corpora- tion- designs, manufactures, sells and services instruments and sup- plies for the healthcare and other industries. Yet the search continues. Coulter scientists constantly seek new ways to apply the Company's knowledge and skill. Areas of particular exper- tise include hematology, chemistry, immunology, particle counting and flow cytometry. The following is a glimpse into the future Coulter's commit- ment to the betterment of mankind. INGIJI 10 THE of rpG cowwit- otni s Γi gniwollot odT form chrowetly. conutive swq include -19qx9 isluciting to 2691A .Ilizle bas to Abbja THE 266K UGM MSAS AGC THE continues Control blies tot THG suq orper suq suq snb- non 26112 I COLDOLS- sbbpections suq CTIC united not serviços bns STOU bLoqnces bloq- Isoigolondos1 ni A combsuigs the coLbo- to vlims} $ peesing elqioning edT !? THE Lot the suq gnisis WICLOSCOBIC - Tot JUG buncible Lot OAGL cpills AGUL2 рэль beils ou СОЛГДЕК suq SCI- Issibom IJJOOU quar 9LG pm S ICM of que msul bas noitsnimetnoo 116 of suq the litter - СОЛГДЕВ Isinsubni bas ,Isoinilo ,IGJ OU CONTLEK® sdmbweur IU porbr- GAGLÀ 92h - Ill OAGL rpe - Williom of 21221 916 H3C H H3C H 14 H A B H H H EADY 1dE/DIV Wallace H. Coulter and Joseph R. Coulter, Jr. co-founded the Coulter companies worldwide. As chairman and president (respectively) they continue their leadership roles in the growth and development of the Corporation. "Wfaster make it easier, more reliable than ever before to diagnose disease. The patient will be the ultimate beneficiary." Wallace H. Coulter 1 Genesis The Search Begins. The year was 1947. In a private counting and sizing microscopic demands and problems of the labo- home laboratory, during his free particles. Indeed, it is estimated that ratory environment. Over the years, time, Wallace Coulter built elec- 95% of all blood cell counters in use with countless new ideas and dis- tronic devices and experimented today have been made by Coulter or coveries, Coulter has demonstrated with a variety of novel ideas. One, are copies based on the Coulter its ability to meet the strictest in particular, would lead to the first Principle. Not only was the princi- requirements. viable method of counting and ple the cornerstone for a company Daily, COULTER instruments sig- three-dimensional sizing of micro- - Coulter Electronics - it also nificantly impact the detection, scopic particles the Coulter launched an industry automated treatment and prevention of disease Principle. hematology instrumentation. in such areas as: early detection of Wallace Coulter's discovery made it possible, for the first time, to count and size biological cells or industrial particles at a rate of sev- eral thousand per second. This achievement earned him two of the Tin Chicaso,ing started highest awards given for scientific corner of excellence. In 1960, he received the John Scott Award, which recog- my basement. Today, Coulter nizes an individual whose invention has benefited mankind. Other provides the means to diagnose recipients of this award include: Thomas Edison, Marie Curie, Gug- disease all over the world. lielmo Marconi, Orville Wright and Wallace H. Coulter Jonas Salk. In 1980, the Institute of Electrical and Electronics Engineers, Inc. pre- This discovery changed the prac- leukemia by rapid, accurate white sented him with the Morris E. Leeds tice of medicine. Now, in a matter blood cell differentiation; diabetes Award for "an outstanding contri- of minutes, patient blood analysis control, through faster and more bution in the field of electrical results are available to the physician accurate glucose analysis; T/B cell measurement." to indicate or confirm a diagnosis. analysis and tumor marker studies Treatment can begin immediately. in cancer research and treatment. Years of experimenting. From a single moment of discov- Before the honors, came the Innovative response. ery in humble surroundings, an experimentation. It was five years Coulter, as a company, is unique innovative corporation has risen to before the first commercial instru- in its diversification; it matches tech- take its place among the world's ment was produced and a pat- nology to the needs of medicine. As leaders in healthcare. ent for the principle secured. Today, an example, clinicians and research- the Coulter Principle is the most ers have a variety of techniques in widely used method in the world for cell analysis from which to choose. Coulter understands the urgencies, 2 ROOM EMERGENCY 3 98 EMERGENCY 215 34 59' Fig. 5 17' 280 20. 70 Coulter People Searching with a Sense of Future. The search continues worldwide. Over 5,000 Coulter employees to help all Coulter people share new It covers a spectrum of disciplines express their confidence through information on clinical procedures that affect the diagnostic process. personal loyalty and commitment. and applications. Computer experts, administrative More than half boast of 10 or more personnel and service engineers all years of service. Quality people with ideas. work to support Coulter scientists It's Coulter employees, commu- as they seek answers to complex Customers who contribute. nicating with customers - teach- medical diagnostic problems. Coulter customers are the most ing, learning, sharing experiences- - important (and largest) group of that make COULTER products spe- People with pride. Coulter people. For many, the asso- cial. Indeed, Coulter's success has It's Coulter people - all over the ciation started in the classroom. come from its ability to translate cus- world - working with common There, they learned to count cells tomer needs into viable products. goals, who have kept the Company on a COULTER counter. When they Throughout the world, Coulter people design, manufacture and dis- tribute diagnostic systems for over "The success is teamwork. real secret to our 80 percent of the world's clinical laboratories. Coulter's total system approach provides its customers with quality products and services. When you walk into a room you can feel it. Joseph R. Coulter, Jr. at the leading edge of technology. moved on to hospital, research and Each person contributes to product industrial laboratories, again, there success and to the continued success were COULTER counters. And the of the Company. Each new product association continues: with Coulter is the result of a team effort. These operator training programs in Coul- efforts are reinforced by the com- ter classrooms, with manuals and mitment of management to further audio-visual programs, and with strengthen the Company's leader- workshops to help operators share ship role in its chosen areas of work experiences. Also, with semi- healthcare and science. nars where experts share research findings with colleagues; with newsletters and other communiques 4 - C = = 50000 ***** ....... 8093 590 1 1 1.20 70 X I 1:10.1 ⑆⑆ 10.0 N A Worldwide Search The search, begun in Chicago, one chemistry analyzer came from spread around the world. Wallace R&D efforts in England; while and Joseph Coulter found the best another, sold in 72 countries, was scientific, engineering, manufactur- designed in France. ing, sales and service people avail- able. As a result, the Coulter Quality products worldwide. Corporation has been international All COULTER instrument manu- for almost as long as it's been a com- facturing facilities produce systems pany. Coulter Electronics, Inc. and which meet the highest performance Coulter Sales Corporation were specifications instruments that "The phrase 'Quality Counts' been associated with this company for a long time with good reason. Joseph R. Coulter, Jr. incorporated in 1958. Coulter Elec- set industry standards. Specially tronics, Ltd. was founded in developed COULTER reagents help England a year later. In 1962, fifteen to keep these instruments at their companies were established with- peak performance levels. To insure in five continents, including reagent quality and integrity, manu- Coultronics in France. In addition, facturing sites have been established COULTER products and services are around the globe. available through a network of more Each day, laboratorians in all than 80 distributor organizations parts of the world rely on COULTER around the globe. reagent and instrument systems to produce the diagnostic information Shared efforts quality work. clinicians depend on to make urgent Part of the Company's strength medical decisions. has been its internationality - its ability to share the fruits of indepen- dent research among all locations. For example, the original design for 6 Coulter Worldwide Coulter Main offices and Manufacturing facilities. Coulter Sales and Services offices. Coulter World Headquarters. The Search for Answers Through Biotechnology. In an age of cost containment, In clinical chemistry. The Coulter tradition of combin- speed and accuracy in medical diag- COULTER systems analyze ing disciplines to solve medical nosis is often the key to a successful patients' blood, providing doctors problems is clearly seen in this clini- treatment regime. Coulter's techno- with rapid diagnostic or therapeutic cal area. COULTER monoclonal anti- logical leadership is a significant drug monitoring information. bodies and flow cytometry systems factor in reducing costs while Hundreds of assays per hour, for a are used together to quickly and improving patient treatment. variety of tests, are performed auto- accurately produce complete, con- Indeed, in this area, COULTER instruments have consistently led the way for the entire industry. The routine clinical laboratory, where once only basic tests were performed, now produces sophisti- O ur strength lies in the cated tests, formerly reserved for breadth of our technical research laboratories. Coulter has made a significant contribution to talents in our ability to solve this transition. problems." In clinical hematology. Wallace H. Coulter Automated COULTER systems produce hundreds of complete blood analyses every day. It would matically, accurately, reliably and cise patient immunology profiles. require several technologists, work- cost effectively. Also, by combining advanced ing full time to keep up with the out- laser technology with two proprie- put of just one of today's COULTER In clinical cellular immunology. tary technologies - the Coulter counters. In hospitals, medical cen- Clinicians need powerful tools to Principle, and high frequency con- ters, and reference laboratories, meet the challenges of diseases such ductivity analysis - cell population COULTER systems detect blood cell as AIDS, leukemia and lymphoma. studies are significantly enhanced. abnormalities which help physi- COULTER flow cytometry systems cians diagnose disease or monitor combine advanced laser and com- In research laboratories. follow-up therapy. puter technologies to examine thou- Coulter has proven its ability to In physicians' offices, COULTER sands of cells per second. Data on find solutions for difficult medical systems produce diagnostic infor- cell structure, size and DNA con- diagnostic problems. And it will mation so rapidly, the doctor has tent is vital in the detection and continue to assist research scientists a patient's results before the exami- treatment of cellular disease. in exploring new frontiers - in can- nation ends. cer detection and therapy, chromo- some analysis for genetic studies, and cellular immunology. 8 Coulter has invested over a dec- ade in laser technology research. The result: Researchers can now sort and analyze cells in minutes instead of months. Similar invest- ments in hematology and immunol- ogy research are opening new areas for investigation. Studies of cell kinetics in both normal and tumor populations are now economically feasible. Other projects investigate characterization of specific cellular enzymes, cell surface markers, 5 hematopoiesis, and tissue transplant rejection. 24 25 H CH2 C H 14 H H 9 The Search Beyond Medicine The result of Coulter technology In process control. is found in diverse fields throughout The success or failure of many the world. Particle size measure- manufacturing operations often ment and characterization are vital depends on the consistency of raw to the production of safe, consistent material particle size. Costly pro- consumer products and to the duction errors and wasted labor are development of new products and technologies. In quality control. Industrial applications include quality control of food, clothing, "Industries Coulter for help over the world paints, perfumes, chemicals, elec- tronic components, biologicals and pharmaceuticals. in making better products. Joseph R. Coulter, Jr. Copiers controlling consistency of the toner. prevented by timely reporting of Chocolate making it smooth vital data from COULTER analyzers and creamy. such as: the size of cocoa bean Paint improving quality of the grindings to produce sweet or bitter pigment. chocolate; mica size for shiny lip- Cosmetics assuring that face stick; polystyrene particles to make powder clings. paint adhere; latex particle carrier systems for monoclonal antibodies. Photographic film allowing more precise light-sensitive emul- In research and development. sions. For almost 30 years, investigators have relied on COULTER instru- Beverages producing quality fil- tration in beer and wine. ments for precise accurate measure- ments to help expand scientific Aerospace analyzing moon knowledge. dust; identifying contaminants in On the frontiers of research, from hydraulic fluids. analyzing moon dust to analyzing Pharmaceuticals assuring the phytoplankton, (unicellular sea correct consistency of tablets. plants, a basic link in the marine food chain), Coulter will continue its commitment to excellence and to the development of the techno- logies of the future. 10 H3C H3C H 14 H3C H I 14 H H A 5 5 5 H H H 3-D PLOT BL DOD PLOT 64-1 L90LS X FLS SF= = 512 TC AN HRESHO OTATE TATE Searching to Better Serve O,, ur customer service begins the moment that someone orders one of our products." Wallace H. Coulter Whether in the research, quality Coulter Education Centers reach control or medical laboratory, all out to every customer. Language Coulter customers share in the specific, hands-on training - con- "proactive" approach to service. ducted by professional instructors The goal is to provide complete and - assures efficient operation of the continuing customer support. most sophisticated systems. Sym- Educating and training every cus- posia, seminars and special publica- tomer is fundamental to Coulter's tions are also available to Coulter success. Virtually every teaching customers. hospital, medical school and re- search laboratory has benefited from Coulter education. Coulter's quality control systems are integral components of cus- tomer support. Laboratories using these systems reach a higher level of performance. In addition, Coulter factory-trained and certified techni- cal representatives are available whenever needed. 12 Epilogue Coulter continues its commit- ment to excellence by bringing the best instruments, diagnostics and services to the healthcare industry and to other industries utiliz- ing the same technologies. Future growth will come from Coulter's continued interaction with clini- cians and researchers, who chal- lenge the established disciplines. Seeking solutions will keep the Coulter Corporation at the leading edge of technology in position to improve existing products and to provide the discoveries of tomorrow. This is Coulter's commitment. The search continues Designed by Gary Singer Communications engaliq -Jimmoo 231 edi gnignind vd 03 JASM bas pase утгивлі 9113 of 26 -siliou sinsubni 19/10 01 bas edi gni most come lliw diworg -inilo driw bounition clash odw bns cistre .29nilqiosib 9ds egnel 9ds Hiw enoisulos gnibesl edt JS Confiss noitisoq ni vgolonrber to egbe bas gniteixe 03 to orly abivorq OJ wonomon .Insmimmoo e'mailuo) si sidT continues efT Designeq ph 2!111 Communications We will cation to be our advancement of medical science. We will fulfill our responsibility to the community." Wallace H. Coulter Coulter Corporation Coulter Electronics of Canada, Ltd. Coulter Scientific Japan Ltd. Hialeah, Florida, USA Burlington, Canada Tokyo, Japan Coulter Electronics, Inc. Coulter Electronics Ltd. Coulter Electronics (Kenya) Ltd. Hialeah, Florida, USA Luton, England Nairobi, Kenya Coulter Leasing Corporation Coultronics France, S.A. Coulter de Mexico S.A. DE C.V. Hialeah, Florida, USA Margency, France Mexico City, Mexico Coulter Electronics of New England, Inc. Coulter Electronics GmbH Coulter Electronics Sales of P.R., Inc. Amherst, Massachusetts, USA Krefeld, West Germany San Juan, Puerto Rico Coulter Electronica, S.A. Coulter Electronics (HK) Ltd. Coulter Cientifica S.A. Buenos Aires, Argentina Hong Kong Madrid, Spain Coulter Electronics Pty. Ltd. Coulter Scientific SPA Coulter Electronics, S.A. New South Wales, Australia Milan, Italy Caracas, Venezuela Coulter Electronics Ind. & Com., Ltda. Japan Scientific Instruments Co., Ltd. Rio de Janeiro, Brazil Tokyo, Japan COULTER ® WORLD HEADQUARTERS 600 West 20th Street, Hialeah, Florida 33010-2428 USA 305-885-0131 ID 404689143 LAB 94 10.9 MO # E0 #<07 # BA # <0 2 02 100 200 300 400 RBC 5.00 HGB 15.9 HCT 46.8 MCV 93.5 MCH 31.8 MCHC 34.0 ROW 14.1 4 RBC 50 100 200 300 PLT 277 MPV 8.3 PLT DO 20 FEMTOLITERS o 23 24 CH 0 O 22 12 25 23 24 26 H F CH2-CH2 20 21 20 H H 3 C 23 23 22 22 H3C H3C R 24 € H H H 17 C - 16 C H3C H 14 H3C U 14 H H3C B 14 14 H R H H H A B A, H H 5 5 5 8 $ H H H H 3-D PLOT BLOOD PLOT X FLS SF= и 512 TC 50000 AN= 0 HRESHO PRISM2 2 ind 8 G OTATE $905) 1 70 XII TATE 1:.0.1 5.0 10.0 X X PAN'S 1/ 6/51 16 15 X=90'LS 2 2P64-NO FITC ADDED Y=FALS 5 READY READY Printed in USA (4203217-4C) - COULTER® STKS Hematology System COULTER ® HEMATOLOGY Scanning electron micrograph of densely packed red blood cells in a capillary, the smallest vessel, made up exclusively of endothelial cells. Magnification 600X. The COULTER STKS Hematology System. Simply Superior in Every Way. Whether you're looking for proven-in-use, walk- away technology or superior cellular classification, the COULTER STKS is the only hematology system proven worldwide to be the best at both. Its rapid, global acceptance by over 1,000 hematology labo- ratories in its first year, proves that COULTER hema- tology instruments continue to outperform any and all alternatives. Singular, superior features in a fully- automated system: The only system with FAIL-SAFE: - Positive Sample I.D. - Positive Cassette Position I.D. - Aspiration Integrity Verification. The only instrument with proven auto- sampling robotics. The only instrument performing multi- technology white cell classification on near-native cells, measured directly in a single channel. The only instrument to study over 8,000 white cells for every differential examination. The only instrument with independent, triple apertures for WBC, RBC and platelet enumeration. The only instrument with no routine maintenance. The only instrument to offer 6 workload scheduling options. The only system that automatically separates normal and abnormal reports using the Auto-Reporter 3. The most advanced computer power applied to hematology. 0 to 24-hour sample stability. Backed by an Interlaboratory Quality Assurance Program of over 8,500 participants. The industry's # 1-rated service organization. 1 Coulter Milestones in the Technologic Integration for a Complete Cell Analysisᵀ Concept. 1953 COULTER COUNTER® Model A. 1968 COULTER Model S Series. 1970 Conductivity Patent. 1978 COULTER S-PLUS Series Instruments with whole blood automated platelet counts. 1978 Immunology R&D begins. 1980 COULTER S-PLUS II with automated Lymph % and # and aperture burn circuit. Mab products introduced. 1983 COULTER S-PLUS IV with Histogram Differential: Automated Mononuclears, Lymphocytes & Granulocytes. Advanced Data Management: Automated Operation & QC. 1984 Automated Closed-Vial Sampling. 1985 Clinical Flow Cytometer. 1986 COULTER STKR with Automated Differen- tial Interpretive Report. 1987 Complete Cell Analysis Concept - Inte- grated Hematologic, Immunologic and Flow Cytometric Analysis. Incorporation of differential capability onto T-Series Instruments. 1987 VCS Technology to study volume, conductivity, light scatter and opacity of white blood cells in their near-native state. 1988 COULTER Q-Prep Immunology Work- station for standardizing sample prep in immunophenotyping. 1989 Introduction of the COULTER STKS System to provide a complete hemogram and dif- ferential in a fully-automated, walkaway, high-volume analyzer. Introduction of the COULTER DNA-Prep System for standardiz- ing sample prep in DNA content analysis. 1990 COULTER™ CYTO-TROL™ Control Cells, the first in vitro diagnostic control for flow cytometry. 1991 COULTER MAXM System. 2 Scanning electron micrograph of blood cells in a venous sinusoid in bone marrow, magnified 1,000 times. In addition to the developing red and white blood cells, the elongated cell in the lumen is a pro- platelet ribbon of cytoplasm from a megakaryocyte. Fat cells are rendered yellow. A small arteriole is seen crosscut at upper left. Singular, Superior Features on the STKS System. Unloading Bay - complete, Triple Apertures for maximum Loading Bay - accommodates identified and audited samples throughput and counting accuracy. 144 samples. are positively linked to cassette position. COULTER FAIL-SAFE features: Self-Cleaning Blood Sampling Laser Bench. - Sample tube and cassette Valve requires no maintenance. position bar codes read at Hydrodynamically focused VCS time of aspiration. Proven Auto-Sampling Robotics Flow Cell for volume, conduc- - Dual Blood Detectors ensure assures continuous sample tivity, light scatter and opacity aspiration integrity. processing and technologist measurements. Autopurge and protection from aerosols. Autoclear ensure flow cell integrity. 4 High Resolution Scatterplot - Comprehensive sample information presents a variety of displays to view discrete white cell populations. Each display represents a differ- ent view of the 3-dimensional plot as it is rotated on its axis. Graphic Printer with color or black and white customized report capabilities. A COULTER Data Management System - Versatile, friendly PC offers help screens and overlapping "window" menu screens for easy operation and training; color displays for all screens; plenty of storage capacity; and open-ended, add- on potential. 5 High-Productivity Sample Processing. MEMATOLOGY 1. Identify samples and tickets through 5. Positive sample I.D. Bar code is read on bar coding. sample tube and linked to bar-coded cassette position at exact time of aspiration to eliminate error. 2. Load samples randomly into 6. Retrieve finished reports automatically 12-unit cassettes. separated into normals and abnormals from the optional Auto-Reporter 3. 3. Stack in STKS loading bay. 7. Stats in secondary mode for micro- sample volumes. Stats may also be analyzed in closed-vial sampling mode using single cassettes. You can interrupt auto-processing at any time for stats without losing data. 7 4 5 6 1 2 3 0 ID 4. Press Start. The STKS begins to produce results in less than one minute. In one hour, find 109 samples fully analyzed, including differentials. 6 User-Defined, Sample Processing and Workflow. Walkaway sample processing - safe and simple. The STKS features Coulter's proven-in-use sampling robotics that ensure thorough sample mixing and unattended sample processing. The STKS loading bay accommodates 144 tubes in a 12 X 12 cassette configuration. Once processing begins, all instrument functions are computer- monitored, including bar code reading and match- ing to sample reports, sample integrity checks, and automatic extended counting for cytopenic samples. Results are even separated into normals and abnormals in the Auto-Reporter 3. You can easily retrieve abnormals for follow-up. The system is extremely easy to run, and requires only 4 reagents, plus COULTER CLENZ® Cleaning Agent. Even system calibration is automated by the computer, and a service call is not required. Flexibility in Workflow Management. The STKS offers you 6 different options for workflow handling: 1. Real-time autoscheduling - using bar code labels. - using cassette position. 2. Pre-assigned worklist with demographics - done manually. - done by the host LIS. 3. Post-assigned worklist. 4. Post-assignment with demographics via Data Base. Other features that make the STKS totally walkaway: Positive sample I.D. Proven auto-sampling - over 3,000 installations. Dual aspiration detectors, placed before and after the blood sampling valve, ensure sample integrity. Continuous computer monitoring of instrument performance. 7 Flexibility in Reporting. The latest software enhancements on the STKS System provide flexible reporting options to meet a variety of laboratory needs. Ability to edit reports and comment on patient files. User-Definable Patient Report provides a chartable report from the graphic printer or the traditional ticket printout. Color-coded workload recording with graphic plotting. Worklist Integrity Checks capture manual transcription errors. Expandable, User-Definable Patient Demographics. Sophisticated QC - simple to use, easy to understand. Over eight sample identifiers for additional security: 1. Positive bar code sample I.D. 2. Patient name. 3. Automatic sequence #. 4. Positive bar code cassette position. 5. Date and time of collection. 6. Date and time of processing. 7. Date of birth. 8. Additional patient demographics. You'll get an excellent report. The Coulter Hematology Cell Classification provides the most specific, sensitive cell classification report available. It includes white cell, red cell and platelet abnormalities and also quantifies (+,++,+++) anisocytosis, microcytosis, macrocytosis, hypochromia and poikilocytosis. This improved cell classification saves labor - by reducing the number of manual differentials - and helps the operator focus on areas of concern on the peripheral blood smear to increase their efficiency in confirmation of abnormalities. Definitive flags and high and low action limits are laboratory adjustable. 8 Chartable Report. Laboratory Report. 02/05/91 10:48:53 DC EDWARDS M.D. 02/04/91 16:53:22 DC EDWARDS M.D. GENERAL HOSPITAL GENERAL HOSPITAL N31256 OPR West Main Street N31256 OPR West Main Street (555) 555 1234 Anytown, Anystate (555) 555 1234 Anytown, Anystate WBC RBC Date of Birth 04/13/1943 Sex M User field ESR RETICS Location MEDICAL 3 1 2 User field FERRITIN TESTS Physician HURST R.R. User field 3 REQUESTED V REL# Date & Time 12/04/90 06:55 Comments ANEMIA OF UNKNOWN ORIGIN RESULTS TO PHYSICIAN ASAP 0 ID# 1 89189876691 WBC 3.8 RBC 4.25 ID# 2 ADAMS, SAMUEL L % # HGB 11.0 50 100 200 300 fL Sequence # NE 2.4 HCT 33.6 FFFFF U LY 27.3 1.0 L MCV 79.0 DATE: 12/04/90 MO 6.7 0.3 MCH 25.9 TIME: 07:07:26 EO M 2.6 0.1 MCHC 32.8 PLT Cass/pos 002001 BA 1.1 0.0 RDW 13.2 E 3 Abnormal WBC Pop PLT 281 REL# Abnormal RBC Pop MPV 9.1 Normal PLT Pop DF 1 2 10 20 30 fL ID# 1 89189876691 4 WBC 3.8 ID# 2 ADAMS, SAMUEL 8 RBC 4.25 % HGB 11.0 L Sequence # NE 62.2 2.4 HCT 33.6 L LY 27.3 1.0 L MCV 79.0 L Date: 12/04/90 5 MO 6.7 0.3 MCH 25.9 L Time: 07:07:26 EO 2.6 0.1 MCHC 32.8 L Cass/pos 002001 BA 1.1 0.0 RDW 13.2 10 Abnorma WBC Pop Comments PLT 281 Abnorma RBC Pop 6 7 ANEMIA OF UNKNOWN ORIGIN MPV 9.1 Normal PLT Pop RESULTS TO PHYSICIAN 9 ASAP 1. WBC scatterplot. 6. Sample status. 2. RBC histogram. 7. Comment field. 3. Platelet histogram. 8. Differential results 4. Sample I.D. and in # & %. demographics. 9. RBC and plt results. 5. Date and time of 10. High and low action flags. sample analysis. Suspect and Definitive flag information is operator selectable. Coulter Classification Chart WBCs RBCs Platelets Instrument-Defined Immature Grans/Bands Nucleated RBCs Platelet Clumps Suspect Classifications: Variant Lymphs Dimorphic RBC Giant Platelets (Lab Report Only) Blasts Population Micro RBCs/RBC Review Slide Fragments RBC Agglutination User-Defined Leukopenia Anisocytosis Thrombocytopenia Abnormalities: (Quantitative +, ++, +++) Definitive Flags Thrombocytosis (Lab Report Only) Leukocytosis Microcytosis Large Platelets (Quantitative +, ++, +++) Neutropenia Small Platelets Macrocytosis Neutrophilia (Quantitative +, ++, +++) Lymphopenia Hypochromia (Quantitative +, ++, +++) Lymphocytosis Poikilocytosis (Quantitative +, ++, +++) Monocytosis Anemia Eosinophilia Erythrocytosis Basophilia Pancytopenia User-Defined All WBC Parameters All RBC Parameters All Platelet Parameters High and Low Action Limits (Chartable Report Only) 9 Help Instructions Select CBC + Diff or CBC Run Controls Analyze Control Print Control Delete Control Help Instructions Select & View File Control Analysis Review/Print Control Print Control Files Delete Control Restore Control Help Instructions Review Graphs Select & View File Print Graphs Help Instructions Control Files Action Limits Definitive Limits Set Up Functions XB Limits *Display Formats *Select Primary Identifier(s) *Customized Patient Report Reagent Lot No., Exp. Date Communication Interface Protocol Help Instructions Maintenance File Repair File Reproducibility Test Special Functions System Diagnostics Carryover Test Auto-Reporter Test *Workload Recording Print Files Modem Data Link Service Help Instructions Reproducibility Test Calibration Carryover Test Auto-Verification Auto-Calibration 12 User-Friendly Interface. Help Instructions CBC or CBC + Diff Print Ticket Run Patients *Print Customized Report Display DF2, DF3 Views Display Single Aperture Data Go to the Data Base Help Instructions Save Patient File Sample Analysis Data Base Query Recall Patient File Batch for Print or Host Transmission *Edit Patient File: demographics, results, comments *Review Data while Batch Processing Delete Data Base Help Instructions Real-Time Auto-Scheduling Bar Code Label *Cassette / Position *Pre-Assigned Worklist (includes Schedule Work demographics and comments) *Manual *By Host LIS (bi-directional) Post-Assigned Worklist *Post-Assignment via Data Base Bar Code Label Print Sequential Number Print Unique Number Help Instructions Patient XB View Current Batch and Mean View Graphs Help Instructions Auto Start-up & Shut-down Print Documentation Log *Repeat Background Count * Available with optional DMS Enhancement Software Package 11 Lightning-Fast Data Acquisition and Storage. The superior Data Management System of the VGA STKS gives you the most advanced computing Graphics power applied to hematology. Support Powerful 16 MHz 386 Microprocessor 32-bit computer platform 4 MB on-board RAM Multiple Math Coprocessors High performance disk subsystem 40 MB hard disk drive 3.5" 1.44 MB Floppy Drive Full alphanumeric keyboard Single button start-up Set user-defined flags. initiates a full system REAGENT DATES TEST 03/31/89 limit limit limit limit Access workload report. check, while you HCT HCT walk away. MCV MCV MCH 22.57 29.52 PCT VDC 11.8 PDW 1. Start-Up 5. User-Defined Flags Comprehensive quality Selectable graphic printer FILE control program for RESULTS format provides backup accurate results. report to ticket printer or Provides 15 controls files to host LIS. of 100 lines each 13.9 Format a chartable report - Trend Analysis Character as a primary report. Windows for Controls Format a report just for (Shown). laboratory use. - Levey-Jennings Graphs. ALL RESERVED F1-Help F4-Print - XB Analysis. 2. Controls 6. Format Customized Report Worklist generation Customize primary and provides flexibility Cass/pos Sequence Current Entries secondary identifiers to in workflow. Cass/pos Status Primary match your workflow. IDENTIFIERS Choose a primary identifier and select from 1 buffer a wide range of optional secondary identifiers. STKS matches sample ALL RESERVED against tube bar code and/or cassette position 3. Sample Analysis 7. Positive Sample I.D. number. and Security Data Base Query pro- Easy interface to link vides immediate access JAMES uni- or bi-directionally Cass/pos to 1,000 patient results, Status Birth Timeout (secs) to host LIS. including all displays. Time Parity ASTM protocol helps Patient results and demo- Stop protect many labs against Edited data Handshake graphics can be edited. Block 258 major software rewrites Data Base sorting and for host computer interface. batch processing Press (7P115) improve laboratory F1-Help F2-Choice efficiency. 4. Data Base 8. Interface 10 Intuitively-Designed Data Management Software. STKS software is easy-to-use. Powerful DMS allows easy software upgrade capabilities. User-friendly software with help screens reduces training time. Pull-down and pop-up menus eliminate confusion of where you are in the software. User-friendly color graphics and simple data formats make information easy-to-interpret. Alpha entry of patient name with alphanumeric keyboard provided. It's power with a purpose. STKS software allows you to sort by name, I.D. number, date/time, flag status, plus batch printing and batch transmission to the host. Automated Quality Control package provides easy printout of all QC information for regulatory compliance. Improve laboratory productivity via multiple tasking. You can review patient or control data while batch processing. Workload Recording, a graphic management tool, assists with resource allocation and cost management. Hard copy printouts of all start-up, patient and commercial control files, and calibration files simplify record-keeping for regulatory compliance. 13 VCS Technology for Automated Differentials 24 Hours a Day. VCS Technology to determine the white cell differential, studies over 8,000 cells in their near-native state for each differential report. All measurements are taken in a single flow cell in which each cell is hydrodynamically focused to ensure it correctly intersects the optical path. Three different measurements are taken simul- taneously by independent technologies. This 3-dimensional study of cell volume, conductivity, and light scatter characteristics also allows for the determination of cellular opacity. Over 16 million data points can be plotted for analysis within the 3-dimensional matrix on each sample - to provide the most complete white cell profile possible. 14 Near-Native State White Cell Analysis. 1. WBCs are analyzed in their near-native state - 4. Only COULTER VCS Technology determines without shrinking or staining - so they retain cellular opacity, a value derived by eliminating their substance, depth and complexity. volume from the conductivity measurement. This allows an even more accurate measure- ment of cellular content. 2. Cell volume is determined using the Coulter 5. Laser light, using forward angle scatter, deter- Principle of Impedance. mines cell surface characteristics, morphology and granularity. 3. Cell conductivity is determined using a 6. Taken together, these measurements provide high-frequency electromagnetic probe, the most comprehensive study of white cell which provides information on the cell's characteristics for accurate classification. internal constituents. 15 Superior-Resolution Scatterplots Allow Visualization of 5 Distinct Normal Populations. By plotting each cell according to its volume, conductivity and light scatter characteristics, each cell can be placed in a 3-dimensional grid. This clustering of cells, based on like characteristics, clearly separates normal cell populations. The data is presented as a 3-dimensional scatterplot which can be displayed on the instrument computer terminal from three different views. Neutrophils Monocytes Lymphocytes Eosinophils Basophils (behind lymphocytes) Population density on the scatterplot is color- coded as follows: Highest density yellow - 11 or more cells. red - 5 to 10 cells. green - 2 to 4 cells. Lowest density blue - 1 cell. By color coding density, rather than cell types, abnormal shifts in population concentration can be identified easily. 16 The DF1 display shows lymphocytes, monocytes, neutrophils and eosinophils. Basophils are behind the lymphocytes. WBC Mo Ne Eo U 0 L U Ly M E The DF2 display shows lymphocytes, monocytes and granulocytes. Mo Gr Ly The DF3 display allows visualization of the basophil population which is hidden from view behind the neutrophils, in the DF2 position. Mo Ba Ly 17 High-Resolution Identification of Abnormalities and Subpopulations. Familiarity with a normal scatterplot pattern Blast cells primarily lymphoid appear in allows technologists to identify the presence of LYMPHOCYTE gated region. abnormalities and subpopulations quickly and Blast cells primarily monocytic appear in easily. In addition to comprehensive white cell MONOCYTE gated region. flagging criteria, the scatterplot presents a Blast cells primarily myeloid appear in visual pattern through which abnormalities NEUTROPHIL gated region. and subpopulations can be detected. Left shift cells appear in NEUTROPHIL Classification of abnormal cell types is made easy gated region. because abnormal cell types fall in their respective gating areas which are individualized for each The diagram below is intended as a guide for analysis. For example: technologist review and data interpretation. WBC 9 2 0 L U 718 M E 3 6 5 4 DF 1 1. Suspect Blasts 4. Giant Platelets 7. Suspect Blasts 2. Suspect Immature Granulocytes 5. Nucleated Red Blood Cells 8. Variant Lymphocytes 3. Aged and Damaged Neutrophils 6. Variant Lymphocytes 9. Suspect Blasts 18 Normal DF1 Examples. Abnormal DF1 Examples. WBC WBC U U 0 0 L L U U M M E E DF 1 DF 1 Identification of immature granulocytes in neutrophil region. WBC WBC U V 0 0 L L U U M M E E DF 1 DF 1 Identification of blast cell population in lymphocyte region. WBC WBC U C 0 0 L L U U M M E E DF 1 DF 1 Identification of eosinophilia. 19 Perfect for Red Cell Measurements. Perfect for Platelet Measurements. PLT RBC Normal Normal REL# REL# 50 100 200 300 fL 2 10 20 30 fL High resolution histograms mean high sensitivity. High resolution histograms optimize platelet Optimal resolution is achieved across 256 channels. population resolution across 64 channels for maximum sensitivity. RBC REL# 50 100 200 300 fL 2 10 20 30 FL Sickle cell disease in crisis: marked RBC Unique, patented, log-fitting platelet algorithm fragmentation, elevated RDW and high take- ensures accurate counts in presence of schistocytes off of the histogram. and microthrombocytes (arrow) 2 10 20 30 FL Unique Pulse Editing Technology eliminates abnormal the algorithm also eliminates RBC interference pulses to ensure size measurement integrity. and captures macrothrombocytes (arrow). Triplicate counting eliminates random error and Unique Sweep Flow Technology prevents red cell ensures precision. Extended analysis periods recirculation, ensuring platelet count accuracy. provide excellent accuracy on cytopenic samples. 20 Coulter Aperture Technology for WBC, RBC and Platelet Studies. The Coulter Principle Remains the Reference Method. We are often asked why Coulter still uses Aperture Technology instead of another means of measure- ment. Our answer is simple - because no other technology has been shown to be superior. It works for red cell and platelet measurements as well as for white cells And it remains the world reference method for counting and sizing blood cells. Platelet Counting Accuracy Using Coulter's Proprietary Algorithm. To ensure platelet count accuracy, the STKS System uses Coulter's patented log-fitting platelet algorithm. Platelets are distributed across 64 channels. The algorithm eliminates RBC inter- ference while capturing macrothrombocytes. You can obtain accurate platelet counts on the STKS even in the presence of small platelets and schistocytes. Triplicate Counting, A Coulter Exclusive. The STKS provides three simultaneous and inde- pendent counts for WBC, RBC and PLT using 3 apertures. These counts are statistically analyzed for agreement - then averaged. Agreement between two of the counts is required for valid averaging. A display is provided for all three counts for each parameter. System Enhancements Ensure High- Quality Quantitative Results. Sweep flow technology, proprietary to COULTER instruments, enhances platelet count accuracy by preventing red cell recirculation. Pulse editing ensures red cell volume accuracy by eliminating abnormal pulses from the analysis which can be caused by cells traversing the aperture in an improper orientation. 21 This Easy-to-Use System Requires Very Few Reagents. COULTER ISOTON® III Diluent. For accurate cell counting and sizing, ISOTON III stabilizes cell membranes while forming the sheath flow in the flow cell to present white cells to the optical path in perfect alignment. COULTER LYSE S® III diff Lytic Reagent. Quickly and thoroughly lyses red cells, while providing accurate hemoglobin measurements. Its use minimizes the need for extra reagents where other COULTER instruments are also in service. COULTER SCATTER PAK. This dual-purpose reagent system within the SCATTER PAK is the key to evaluating white cells in their near- native state. Erythrolyse II lyses red cells and dilutes the sample. StabiLyse preserves the structure, surface characteristics and size of the white cells for the volume, conductivity, opacity and light scatter measurements. COULTER 4C® PLUS Cell Controls. Extreme sensitivity and tightest range of expected values ensure the highest precision and accuracy. 4C PLUS controls set the industry standard for controlling CBC parameters at clinically- significant low, normal and high levels. COULTER LATRON Control. Assures the accuracy of the 3-dimensional measurements of volume, conductivity and light scatter for instruments using VCS Technology. It checks for proper gain, capacitance and laser flow alignment. COULTER S-CAL® Calibrator. Provides single point values - traceable to reference methods - for accurate instrument calibration - without a service call. It's also a System that Protects Technologists from Biohazards. Closed-Vial Sampling. In the primary mode, you'll never pop a top. The instrument's sam- pling needle requires no maintenance and is self-venting. Safe Reagent System. You'll never be bothered by reagent neutralization. With no formaldehyde and minimal cyanide, you can discard reagents without labor-intensive handling. Cap-pierceable controls and calibrator. COULTER 4C PLUS cell controls and S-CAL Calibrator can be used in the closed-vial sampling mode to further protect technologists from potential biohazards. 22 4C PLUS 20 LITERS COULTER CELL CONTROL (5.3 gallons) LECTROLYTE SOLUTION COULTEi TON III ⑉ For in Vitro Diagnostic Use - HEMATOLOGY REFERENCE CO us NO 4,299 726 and you PROTECT FROM FREEZING CBY SI Active Ingredient: Stabilized tot Ht BIONAZARDONS MATERIAL no DES this - by on the to be COULTER PN 7546771 DO NOT by STACK OVER COULTER DIAGNOSTICS 4 HIGH Hickion R 33014 7502182-A R 6.90 SCIENTIFIC INC. Houston, GENT 1 $ - S COULTER LATRON CONTROL MENTS OSCARD I I CAUDON - E A OULTER 7546917 1520 # ERYTHROLYSE . S-CA An erytheots bytte reagent INCREDIENTS - 03-09 at Technologic Advancements Eliminate Maintenance Procedures. CMS #257-521 10 LITERS COULTER #8546931 COULTER CLENZ cleaning for components COULTER CLENZ COULTER CLENZ Cleaning Agent maintains the integrity of the system's highly-sensitive flow cell, counting chambers, apertures, blood sampling valve and Hb cuvette. Shown above are examples of an aperture, before and after cleaning with COULTER CLENZ Cleaning Agent. A key to our claim of "No Routine Maintenance" is Coulter's patented blood sampling valve which is constantly maintained, automatically. Coulter's patented burn circuit means the end of protein buildup and the end of routine aperture maintenance. No Routine Maintenance. Maintenance Daily Weekly Monthly BSV Cleaning NO NO NO Aperture Cleaning NO NO NO Needle Replacement NO NO NO Tubing Maintenance NO NO NO Protein Removal NO NO NO Calibration NO NO NO Fan Filters NO NO YES Syringe Cleaning Not Applicable Not Applicable Not Applicable Flow Cell Cleaning NO NO NO 24 No Routine Maintenance. Labor-saving features give you more time to run patient samples. You can lower labor and operating costs, as well as instrument downtime. Forget routine replacement of apertures, baths, chambers, needles, tubing or pumps. Here are some of the maintenance-saving features that make this instrument the least labor-intensive analyzer on the market today. Self-Cleaning Blood Sampling Valve. Never remove it. Constant circulation of ISOTON III diluent between each sample, and COULTER CLENZ Cleaning Agent during shut-down, keeps the sampling valve clean and fully-operational at all times. Patented Burn Circuit. Automatically removes protein buildup, once and for all. With virtually no maintenance, your apertures will stay clean and protein-free to ensure the accuracy of RBC parameters, including MCV. Non-pinch tubing. Forget about manual main- tenance. Routine tubing replacement is a thing of the past. Autoclear and Autopurge. Flow cell integrity is constantly maintained by these two computer- controlled functions. The instrument knows when rinsing and purging are required and carries it out automatically, without you. Dual Blood Detection System. Measures the optical density and timing of the sample, before and after traversing the blood sampling valve, to ensure consistent sample integrity. COULTER CLENZ Cleaning Agent. Routine use eliminates the need for system bleaching while maintaining all fluidics and optical pathways. 25 Coulter QC and Support - The Best in the Industry. Coulter's Interlaboratory Quality Assurance Program (IQAP). Coulter has always prided itself on the personalized service it gives its customers. This is certainly true of IQAP. Over 8,500 laboratories receive monthly reports comparing their system performance to other labs using the same COULTER instruments and lot numbers of 4C PLUS cell controls. IQAP helps your lab eliminate some of the time- consuming recording and computation steps needed for a qualified quality assurance program. It's an easy-to-read report on accuracy and pre- cision that helps satisfy the requirements of state and regulatory agencies. QCC - It's Just for Quality-Conscious Customers. By choosing COULTER instruments, reagents, controls, calibrators and service, we know you care enough to deserve extra benefits and values that aren't available to others. That's why Coulter has designed its QCC Program - - to provide Coulter customers with special advantages. QCC can help relieve continuing education costs and keep your library current with publications. QCC. It's just our way of saying "thank you" for being a Quality Coulter Customer. Coulter Quality Includes Customer Service, Support and Training. For six consecutive years, the Coulter Service Organization has ranked #1 in the industry.* And we intend to keep it that way. That's why we back your COULTER STKS instrument with our exclu- sive "95% UP-TIME GUARANTEE." Coulter offers many service options so you can be sure you get exactly the kind of coverage you need. It's the most extensive array of service options in the industry. The Coulter Education Center in Miami Lakes, Florida, ensures that when you return to your laboratory you get the most from your new instrument quickly. All customers get language- specific, hands-on training. *1990 IMS SERVICETRAK data. 26 When You Choose COULTER Products, You Choose the Company Behind Them. ACIAC I I I I I I I I I I into I I To join IQAP, you merely need to use 4C PLUS To keep our service rating #1 in the industry, cell controls on a regular basis. Then you can Coulter trains over 600 service representatives per submit data from any number of COULTER year on all products and all product lines. With instruments, from any number of labs (main over 1,350 technical representatives worldwide, lab, stat, satellite), and from any number of shifts. a Coulter Service Representative is always there IQAP is an easy and efficient way to monitor the when you need one. on-going precision and accuracy of all your hematology instruments. QCC Gandelines QCC Catalog Code Quality Customers Count Use your QCC Value Points for tuition on courses The Coulter Education Center in Florida is staffed at the Coulter Education Center and Coulter Field with 19 instructors who train over 2,400 customers Training Centers; in-laboratory training courses or a year. They teach hematology, chemistry and Outreach programs; admission to regional seminars, flow cytometry, including applications such as workshops and symposia; or to obtain technical immunophenotyping and DNA analysis. and educational publications and AV programs from Coulter. With QCC, you're also eligible for service contract enhancements. 27 Coulter Complete Cell Analysis Case Study. Hematologic Presentation. WBC A 7-year-old white male was investigated to rule RBC out rheumatic fever. A CBC and differential U REL# was requested. 0 L 50 100 200 300 FL U The CBC showed a leukocytosis with a pre- M PLT dominance of cells in the lymphoid region. E REL# The instrument cell classification reported the suspected presence of blasts and variant DF 1 2 10 20 30 fL lymphocytes, along with a relative neutropenia ID#1 653738 WBC 13.8 H RBC 3 88 L - ID#2: X # HGB 9.2 1. and absolute lymphocytosis. NE 23.3 L 3.2 HCT 28.2 L Date: 12/06/90 LY 71.1 9.8 H MCU 72.5 L Time: 08:56:28 MO 4.4 0.6 MCH 23.7 L 80 0.3 L 8.0 MCHC 32.6 L The red cell profile showed a moderate microcytic Cass/Pos:0004/02 BA 0.8 0.1 RDW 13.5 R hypochromic anemia. Abnorma WBC Pop PLT 27 RL Abnorma RBC Pop MPV 7.9 R Abnorma PLT Pop The platelet count was low (27,000) with an FIGURE 1 abnormal distribution. (Figure 1) The peripheral smear (Figure 2) confirmed the instrument findings of moderate anemia, decreased platelets, relative neutropenia and a homogenous population of dysplastic mono- nuclear cells. The bone marrow aspirate revealed increased cellularity with 99% of the marrow elements identified as malignant acute leukemia lymphoblasts. Most cells showed FAB-L1 morphology. These lymphoblasts were alpha naphthol butyrate esterase negative; myeloperoxidase negative; acid phosphatase negative; PAS positive. In this case, the hematologic findings on the peripheral blood smear, along with bone marrow studies and special stains, indicated a probable lymphoproliferative process. Based on these findings, a diagnosis of ALL was made. A further workup was performed for Complete Cell Analysis to determine the cell lineage(s) involved in the proliferative process. FIGURE 2 28 Immunologic Findings. As shown in Table 1, the cells of interest are negative for expression of T cell and Myeloid markers. Greater than 96% of the cells express J5 (CALLA), while 93% are positive for 13, 96% for B4, and 21% for B1. Low percentage markings with all 4 Myeloid markers are consistent with previous results which indicated a lymphoid process. Low markings with T cell antigens T11, T4, T8, T3, T6, versus high markings with B1 (21%), and B4 (96%), indicate B cell line proliferation. TABLE 1 Patient Results Patient Results Description Antibody % Positive Count cells/µl Lymphocyte Markers (T&B Cells) E Rosette Receptor T11(CD2) 11 1661 T Helper Cell T4(CD4) 7 1057 T Supressor Cell T8(CD8) 7 1057 TL T Lymphocyte T3(CD3) 10 1510 Total Thymocyte T6(CDla) 0 0 B Lymphocyte B1(CD20) 21 3171 B Lymphocyte B4(CD19) 96 14496 Myeloid Markers Monocytes, Myeloid Cells Mol(CD11b) 1 151 Monocytes Mo2(CD14) 1 151 Myeloid Cells MY7(CD13) 0 0 Myeloid Cells MY9(CD33) 1 151 Specialty Markers CALLA J5(CD10) 96 14496 HLA-DR I3 93 14043 Figure 3 shows a Co-Plot of selected monoclonals tested in this case. Significant positivity is shown for the following antibodies: 13, B1, B4, and J5. On the basis of these studies, a separate aliquot of cells was processed for DNA content and cell B1 cycle analysis. I3 B4 T3 J5 CONTROL ALL FIGURE 3 DNA Content Analysis. The patient's sample was analyzed on the COULTER EPICS® Profile II Clinical Flow Cytometer for DNA content using propidium iodide for fluorescent GoG, GgG1 staining. Figure 4 shows a normal DNA content U histogram. Figure 5 shows the patient's histogram with an aneuploid peak to the right of the normal Aneuploid Peak G₀/G₁ population (hyperdiploid). In many cancers, G2M aneuploidy is associated with an unfavorable DNA CONTENT DNA CONTENT prognosis and a poor response to chemotherapy. FIGURE 4 FIGURE 5 A notable exception is ALL where aneuploidy has been associated with improved survival in children. 29 Scanning electron micrograph of human blood showing a number of spherical, yellow lymphocytes and two biconcave erythrocytes. The lymphocytes have numerous microvilli projecting from their outer surfaces; the two cells at top center appear to be in the process of division. Coulter Worldwide Sales Offices: Country Phone Country Code Number Australia 61 2-905-0688 Brazil 55 21-342-2358 Canada 416-639-4701 Dubai 971 4-213534 France 33 1-34-27-23-23 Germany 49 2151-818-0 Hong Kong 852 8140481 Italy 39 2-982-951 Japan 81 3-3877-9233 Kenya 254 2-567804 Latin America 305-885-0131 Mexico 52 5-688-74-23 Netherlands 31 2979-88578 New Zealand 64 9-886-621 Portugal 351 1-895828 Puerto Rico 809-752-8420 South Africa 27 11-805-2055 Spain 34 1-6-45-30-11 Turkey 90 4-1402536 United Kingdom 44 0582-491414 United States 305-885-0131 (Ext. 2646) 1-800-526-6932 Venezuela 58 2-52-66-68 ® Registered trademarks of Coulter Corporation ™Trademarks of Coulter Corporation © Copyright 1991 Coulter Corporation All rights reserved. Printed in U.S.A. Subject to change without notice. 4203326-0B Rev. 4/91 4/91 7 8 4 5 1 2 CE 0 9 6 ID 3 . F COULTER COULTER ELECTRONICS, INC. 601 West 20th Street Hialeah, Florida 33010-2432 USA Mailing Address: P.O. Box 2145 Hialeah, Florida 33012-0145 USA Telephone: (305) 885-0131 March 8, 1991 Haseah Ms. Shirley M. Green Special Assistant to the President THE WHITE HOUSE 1600 Pennsylvania Avenue, N.W. Washington, D. C. 20006 Amberst Coulter Buens Ares Argentina Dear Ms. Green: Coulter Electronics Pr. Ltd We have received copies of correspondence between Dr. Penn Lupovich, Director of You South Wales Australia Laboratories for Group Health Association, Inc., in Washington, D. C., and The White House Coulter Electronics Lida in connection with Dr. Lupovich's nomination of Wallace H. Coulter, Chairman of the Board Riode Janeiro, Brazil and Founder of The Coulter Corporation, as recipient of the Medal of Freedom. Coulter Electronics of Canada Ltd Burlington Canada We were very pleased to read Dr. Lupovich's nominating letter and we fully agree that Dr. Coulter is deserving of this great honor. His contribution to the world of medicine, clinical Coulter Electronics Ltd Luton England pathology and scientific and industrial research has been outstanding. Coultronics France SA Margency France Dr. Coulter pioneered the development of automated laboratory instrumentation four decades ago and has guided the many remarkable advances in the development of technological Coulter Electronics GmbH Krefeld West Germany instrumentation we see in the clinical and research laboratory today. Coulter instruments developed from the Coulter Principle, discovered in 1947-48, have played a valuable role in the Coulter Electronics (HK) Ltd major medical and scientific advances of recent years. Hung Kong Counter Scientific SPA Dr. Coulter's background reflects the American spirit of invention in this century. Born Milan Italy in 1913, he grew up during the days when electronics was in its infancy, and among his childhood Japar Scientific Instruments Co. Ltd idols were such geniuses as Edison, Marconi, Bell and other seekers into the unknown and Tokyo Japan untried of their day. Coulter Scientific lapan Ltd Tokoo Japan His father was a railroad telegrapher/dispatcher and his mother a schoolteacher. During his early childhood he moved with his family to various parts of the country, always encouraged Coulter Electronics Kenyal Ltd Nairobi Kenva by books and always free to give vent to his insatiable curiosity. From the beginning, radio and electronics fascinated him. As a youth, he studied both with the intention of making radio and Coulter de Mexico SA DE CV electronics his career. Mexico City. Mexico Coulter Electronics Sales of PR inc Dr. Coulter discovered the Coulter Principle while experimenting in his basement San luan Puerto Rico laboratory/workroom in Chicago during the late 1940's. He was trying to find a way to count Coulter Cientifica SA minute particles, such as blood cells, electronically. At that time laboratory technologists were Madrid Spain conducting blood tests manually, using a microscope -- a time-consuming, often inaccurate Coulter Electronics SA method. Caracas Venezuela Cont'd Page 2 Coulter Quality Counts! 4232099 :8 2 Page 2 Ms. Shirley M. Green THE WHITE HOUSE The Principle led to construction of an instrument that would count blood cells hundreds of times faster and much more accurately than the manual method, and a small industry was spawned in Coulter's Chicago basement. The first COULTER COUNTER analyzers were designed and built one-by-one by Wallace and his brother Joseph during their free time after work, with the help of one full-time employee. Details on the man and his Company can be found in the attached booklet and biography. In recognition of his ground breaking discovery, in 1960 Wallace Coulter was named recipient of the coveted John Scott Award for Scientific Achievement. This was a special honor, because it linked him with such past recipients as his early role models Thomas Edison and Guglielmo Marconi, not to mention the renowned Madam Curie, Orville Wright and other scientific luminaries. That same year his unique invention, the COULTER COUNTER. automated blood cell and particle analyzer was tapped by the Department of Commerce for the distinction of being the first American-made product to be shown behind the Iron Curtain at the 19th International Fair in Plodiv, Bulgaria. Shortly after that it made its European debut at an exhibition in Germany. World wide interest grew quickly, and Wallace and his Principle reached international importance. Today, the Company started by Wallace and Joseph Coulter is wholly owned by the Coulter family, and the same "spirit of family" that existed with their employees during the early years in the Chicago basement facility continues today in Coulter companies around the world. Dr. Coulter continues to play an active role in research and development working with a select group of world class engineers and scientists to expand the Company's product lines to meet the needs of the future. In addition to the John Scott Award, he has been recipient of numerous honors and awards over the years. He holds several honorary degrees. His Principle has been cited as the basis for the first viable modern flow analyzer. Dr. Coulter actively sought ways to make his discovery useful in service to others. Some examples of this are: In the early 1960's a COULTER COUNTER blood cell analyzer was given to the Hospital Ship HOPE in the name of the employees of Coulter Electronics. The People to People Foundation still use the instrument in its efforts to provide medical care for America's poor. In the early 1980's Dr. Coulter donated a COULTER EPICS laser-based cell separator to the US Resource for Flow Cytometry at Los Alamos. Cont'd Page 3 Coulter Quality Counts! 4232050 IC R 4 87 Page 3 Ms. Shirley M. Green THE WHITE HOUSE A COULTER COUNTER particle analyzer was used to study soil taken from the moon and one is currently being used for special blood studies in space. Several COULTER COUNTER analyzers were sent to the Soviet Union, along with reagents and controls, to be used to monitor the health of victims of the Chernobyl disaster. A COULTER COUNTER analyzer was donated for use in the treatment of victims of the Armenian earthquake. Countless U.S. hospitals, medical schools, clinics and research centers have been recipients of Dr. Coulter's largesse. He has funded or supported many research programs, and awarded many grants and endowments. The most recent was the endowment for a lecture series through the Department of Laboratories at the University of California, San Francisco. The Coulter Corporation grew from its humble beginnings in a Chicago basement to become the worldwide leader in clinical laboratory instruments. Today, Coulter has companies and offices in 20 countries - the first was founded in England in 1959. Coulter has sales, service and distribution operations in more than 100 other countries and employs over 5,300 people world wide. While the Japanese were busy buying U.S. companies, Coulter was acquiring their Japanese distributor in order to meet the challenge of Japanese competition. Coulter's Japanese company is a wholly owned subsidiary. More than 50% of the company's business is international and, at this writing, there are more than 58,000 Coulter hematology devices operating in the world, running more than 3,000,000 tests a day. Thousands of Coulter systems and devices for chemistry, fine particle, and flow cytometry analyses are also in use daily around the world. The Coulter Principle has provided the gateway to all subsequent hematological advances. emerged. From the Coulter Principle discovered by Wallace Coulter in 1947, an entire industry has More than 95% of the blood tests done around the world today are conducted with the use of a Coulter device or its clone. Wallace Coulter's discovery and the products that emerged from the Principle are providing the powerful tools being used by clinicians and scientists to diagnose and design treatment for killer diseases such as Aids, Leukemia, Lymphoma, etc. Dr. Coulter's contribution has been considerable. Both the man and his work exemplify the American spirit at its best - ingenuity and inventiveness combined with caring and community service. Cont'd Page 4 Coulter Quality Counts! 4232050 IC R 4 87 Page 4 Ms. Shirley M. Green THE WHITE HOUSE We honor Dr. Coulter for his contributions to the health care of mankind, and we join Dr. Lupovich in urging President Bush to consider him as an honored and worthy recipient of the Medal of Freedom. Sincerely, line Junne Keslan Anne-Lynne Keplar Director of Business Development ALK:PF 90241 encl: WHC Biography Coulter Corporate Brochure Coulter Quality Counts! © 4232050 IC R 4 87 t le Group Health Association, Inc. outgoning Department. of Laboratories, 1709 New York Avenue, N.W. Washington, D. b. 20006 Telephone: (202)383-1800 October 9, 1990 Director of Laboratories Associate Director of Laboratories Penn Supovich, M. D. James D. Mac-Lowng. M. D. The Honorable George Bush President Of The United States The White House 1600 Pennsylvania Avenue, N.W. Washington, D.C. 20006 Dear Mr. President: I should like to take the liberty of nominating Wallace H. Coulter, for The Medal Of Freedom. He is a great American who has contributed in no small way to the health, welfare and economy of our people in ways which represent the best of the capitalist tradition of our wonderful Country. Mr. Coulter is the discoverer of the Coulter Principle and the founder of Coulter Electronics, Inc. of Hialeah, Florida. He is a scientist and engineer of extraordinary talent and accomplishment. When I was a young man in the 1950's doing volunteer work at The Montefiore Hospital in Pittsburgh, Pennsylvania, I remember learning how to do "blood counts" the old fashioned way. We were lucky if we could get the basic information that was needed for the patient's care within forty minutes. When I became a first year resident in pathology at that same institution in 1965, I recall that our laboratory director purchased one of the first models of the original Coulter counters. Because of Mr. Coulter's ingenious invention we were then able to achieve far more accurate information in less than five minutes. To date, evolution of the principal which Mr. Coulter discovered has caused a monumentally beneficial change in the quality of patient care at a cost which has been readily affordable. It is rare that an evolving technology produces that kind of result for not only our advanced, relatively wealthy society but for the rest of the world as well. Any physician in any country today can verify the contribution of the "Coulter Counter". Please know that I have no connection whatsoever with the Coulter organization and I am rather certain that Mr. Coulter would not even know who I am. I feel it to be my duty to try to see that Mr. Coulter receives recognition in his later years by the American people for the incredible contributions that he has made, and in fact, continues to make to this day. He is hard at work in the development of technology which once again is revolutionary and I suspect that a man of his vision has much yet to teach the rest of us mere mortals. Enclosed is a copy of the brief biography which was supplied to me by one of the Coulter Sales staff who has no idea what I have in mind. Also enclosed is a copy of my own C.V. SO that my credentials for presuming this nomination may be known. I ask that you weigh his accomplishments in consideration of nominating him for the Medal of Freedom. Respectfully, Penn LufanD Penn Lupovich, M.D. PL/jeg WALLACE HENRY COULTER Biography Wallace H. Coulter, co-founder and When the Jap-anese invaded Singapore he Chairman of the Board of Directors of the escaped to Java, and from there, for almost world-wide Coulter Corporation, is best noted a year, he island-hopped his way back to as the discoverer of The Coulter Principle, the the United States. most widely used method for counting and sizing microscopic particles suspended in a From 1942 until the end of World War fluid. His method has been called the "first II he worked in electronic development for viable basis for flow cytometry," and from it Press Wireless in New York. He later grew an industry that changed the world of participated in electro-medical diagnostic medical research for all times. instrumentation development for Raytheon Manufacturing Company. Born in Little Rock, Arkansas, Wallace grew up in an era of invention! During his Upon returning to Chicago in 1946, he pre-teens, when radio began coming into its worked for Illinois Tool Works and the own, he was caught up in the fascinating Mittelman Electronics Division of Century new field, building crystal sets and Steel, spending his spare time in his experimenting with electricity. basement workroom building electronic amplifiers and generators for local firms. The fascination continued through his Here he and his brother Joe also spent many school years and carried him to Westminster hours experimenting with a variety of novel College in Fulton, Missouri, and further study ideas including a method for counting of electrical engineering at Georgia Institute microscopic particles. of Technology. It was this activity that finally led him New vistas were opening to adven- to discovery of The Coulter Principle a turous young men, and Wallace, whose role method for counting and three dimensional models included such great inventive genius' sizing of microscopic particles -- a as Thomas Edison and Guglielmo Marconi, technology that was to have a profound was drawn into this challenging new world. effect on the practice of hematology. An avid reader and insatiable The Coulter Principle provided the explorer, problems looking for solution drew much desired and long hoped for means of him like magnets. sizing particles on the basis of volume, the most meaningful single measure of size. His early career in electronics began in 1934 when he took a job as a radio station Using the Coulter Principle, biological engineer-announcer. cells or industrial particles could be counted and sized at a rate of several thousand a In 1937 he joined the General Electric second -- a wondrous feat when compared X-Ray Corporation taking on responsibilities as to the time consuming manual method used sales engineer. by lab technologists with a microscope. G.E. sent him to the Far East, where From the principle evolved an he worked in Manila, Shanghai, Singapore instrument, created with a grant from the US and the nearby islands where he sold and Department of Naval Research for use at the serviced x-ray equipment to local hospitals. National Institute of Health. Wallace Henry Coulter Biography 2 Wallace's first patent was approved cellular enzymes, cell surface markers, in 1953, and he and his brother Joe, an hematopoiesis and tissue transplant rejection. electronic engineer, began the one-on-one production of a commercial instrument, the His expansion of biomedical research Coulter® Counter cell and particle analyzer. efforts in development of monoclonal antibodies and flow cytometry systems Research continued, sales increased, represents the beginning of the integration of and in 1958 Wallace and Joe Coulter launch- three distinct disciplines: hematology, ed a company Coulter Electronics, Inc. immunology and flow cytometry. From the Corporation evolved an Wallace Coulter continues guiding industry development of automation in the explorations of new areas of research of hematology laboratory! nuclear and other intracellular markers to better define cell function. He has also Today, the wholly-owned Coulter expanded his Corporations's research in Corporation has over 5,000 employees oncology, with development of tumor working in manufacturing facilities and sales, markers for diagnosis, prognosis and service and education operations in 20 monitoring patient therapy. companies around the world. The image analysis work of the 1970's In Coulter's wake followed many will complement this work by providing companies that developed instruments using modernized visual measurement in such Wallace's Principle. It has been estimated areas as DNA content and estrogen and that at least 95 percent of the automated progesterone receptor levels. blood counts done in the world today are done on Coulter instruments, or their clones. Wallace Coulter holds several honorary doctorates in science and Always searching for newer horizons, engineering. he used his Principle and the first COULTER® Counter cell analyzer to spawn entire families He has been honored with many of instrumentation and reagents and awards, among them the 1960 John Scott controls, not just in hematology, but also in Award for Scientific Achievement, previously industrial fine particle counting, chemistry, awarded to such notable achievers as and other related lab instrumentation. Thomas Edison, Guglielmo Marconi, Marie Curie, Jonas Salk, and Orville Wright. Such research led to the discovery of valuable new parameters of cell opacity. An Adjunct Professor in the University The combination of The Coulter Principle and of Miami's School of Medicine's Department opacity opened new avenues for cell of Biomedical Engineering, Dr. Coulter is a classification and analysis. member of the Miami Chapter of Sigma Xi. He is a Fellow of the Institute of Electrical In the 1970's the Corporation invested Engineers and was recently named a Trustee heavily in the research of image analysis. of Clarkson University in New York. In the 1980's research was begun in Among the honors he has received immunology, which resulted in products in are: the IEEE Morris E. Leeds Award in 1980; retrovirology, studies in the area of cell the 1988 "Florida Industrialist of the Year kinetics in both normal and tumor Award," a state award presented by the populations, characterization of specific Museum of Science and Industry in Tampa, I Florida; the 1989 M.D. Buyline's SAMME Lifetime Achievement Award; the American Society of Hematology's Certificate for Distinguished Achievement in 1989, and he was the 1989 recipient of the Association of Clinical Scientists' Gold Headed Cane Award.